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How to use Easysweet Amphetamine (AMP) Rapid Test

  The Easysweet Amphetamine (AMP) Rapid Test is a fast chromatographic immunoassay for the detection of methamphetamine in human urine with a cut-off concentration of 1000ng/ml.

  Materials provided by Easysweet Amphetamine (AMP) Rapid Test:

  1. Test device

  The test consists of mouse monoclonal anti-methamphetamine antibody-conjugated particles and methamphetamine protein conjugates. Use goat antibodies in a control line system.

  2. Instructions for use

  Required materials, not provided:

  1. Urine collection container

  2. Timer or Clock

  

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  Methamphetamine is an addictive stimulant that strongly activates certain systems in the brain. Methamphetamine is chemically closely related to amphetamine, but methamphetamine has a greater effect on the central nervous system. Methamphetamine is manufactured in illicit laboratories and has a high potential for abuse and dependence. This medicine can be taken by mouth, injected, or inhaled. Acute high doses enhance stimulation of the central nervous system and cause euphoria, alertness, decreased appetite, and feelings of increased energy and strength. Cardiovascular responses to methamphetamine include increased blood pressure and cardiac arrhythmias. More dramatic reactions can produce anxiety, paranoia, hallucinations, psychotic behavior, and eventually depression and exhaustion.

  The effects of methamphetamine typically last 2-4 hours, and the half-life of the drug in the body is 9-24 hours. Methamphetamine is primarily excreted in the urine as amphetamine and its oxidized and deaminated derivatives. However, 10-20% of methamphetamine is excreted unchanged. Therefore, the presence of the parent compound in the urine indicates methamphetamine use. Methamphetamine is usually detectable in urine for 3-5 days, depending on the pH of the urine.

  Easysweet Amphetamine (AMP) Rapid Test

  The Easysweet Amphetamine (AMP) Rapid Test is an immunoassay based on the principle of competitive binding. Drugs that may be present in the urine sample compete with the drug conjugate for binding sites on the antibody.

  During the test, the urine sample migrates upwards by capillary action. If the methamphetamine level in the urine sample is below 1000ng/mL, the binding sites of the antibody-coated particles will not be saturated in the test. The antibody-coated particles will then be captured by the immobilized methamphetamine conjugate and a visible colored line will appear in the test line area. If the methamphetamine level is at or above 1000ng/mL, no colored line will form in the test line area because it will saturate all the binding sites of the anti-methamphetamine antibodies.

  Drug-positive urine samples will not produce colored lines in the test line area due to drug competition, while drug-negative urine samples or samples containing drug concentrations below the cut-off value will produce line areas in the test line area. As a program control, a colored line will always appear in the control line area, indicating that the appropriate volume of sample has been added and membrane wicking has occurred.

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